CellVysion
The avidity of the antibody to Natural Killer cells and tumor cells.
We wanted to share some exciting insights from the Advanced Immunology conference in Barcelona (September 23/24, 2024), particularly reaching an optimum in the avidity of the antibody to Natural Killer (NK) cells and tumor cells.
The ability to determine the avidity of NK cells binding to cancer cells through therapeutic antibodies is a standout application of the CellVysion. This is a game-changer in selecting the most effective therapeutic antibody based on the avidity of both cell types. The antibody acts as a vital bridge, enabling optimal interaction between NK cells and cancer cells.
For companies developing bispecific antibodies, understanding cell-antibody-cell avidity is crucial. The better these bispecific antibodies function as bridges, the more effectively NK cells and other T-cells can target and eliminate cancer cells. Testing the avidity of the antibody against NK cells and tumor cells is essential for maximizing therapeutic efficacy.
Other biotech and pharmaceutical companies are leveraging various T-cell to cancer cell coupling molecules. With CellVysion, utilizing a capture gradient with CaptureSelect anti-antibodies allows for the dynamic capture of various antibodies, enabling precise measurement of cell avidity through tipping point shifts. Remarkably, the effectiveness of immunotherapy hinges on the strength of these connections, there is an optimum for the avidity, leading to higher efficacy in therapeutic applications.
Our mission is to identify the antibody that binds to cancer cells with the perfect strength within an optimal range. The CellVysion from Vysens is designed to achieve this. Moreover, the CellVysion is a Plug & Play system and all information is available after one single run.
We developed the CellVysion with a gradient system with this purpose in mind. We are very interested to receive your opinion. Of course, if you want to carry out a pilot study with us, please contact us. Let's explore this opportunity together!
As an example we describe measuring the avidity of cells on a ligand density gradient by determining the so-called tipping point for a certain cell type - antibody combination. The tipping point is the characteristic ligand density that is just enough to capture the cells/particles under certain shear conditions. This value of avidity determined as functional Rmax value depends both on the number of receptors and the monovalent affinity per cell. Therefore, this setup and method will allow for cell-antibody avidity determination. Once we determined the tipping point, we measure the ligand density at the tipping point.